Resources
The Stem Cell Core houses 3 cell culture suites, 2 lab benches, and cell analysis. Each cell culture suite is outfitted with 3-4 bio-safety cabinets, 6-8 CO2 incubators, a low oxygen incubator, and inverted microscopes and requisite equipment for stem cell culture. The STEM Core is located adjacent to the Flow Cytometry Core Facility enabling efficient cell sorting and cytometry analysis experiments.
Cell Imaging
2x Dissection microscopes for manual isolation and manipulation of stem cell colonies
SeaHorse 96XFe for cell analysis
Cell Culture
11x Biological safety cabinets
18x C02 Incubators
3x N2/C02 Incubators (hypoxic conditions)
3x Bench top centrifuges
3x 37°C Water baths
Transfection
LifeTech Neon – Tip based electroporation (adjustable parameters)
MaxCyte ExPERT A+X electroporator (Cuvette-based; fixed parameter)
Centrifugation
Optima L-80XP Ultracentrifuge
Allegra X-15R Plate centrifuge
Bench top temperature controlled centrifuge & microcentrifuge
Refrigerators and Freezers
3x -80°C freezers
10x -20°C freezers
6x 4°C refrigerators
2x High capacity deli refrigerators
2x MVE 800 Series Cyrostorage freezers – 11664 vial capacity, auto fill controllers
