Overview
Genetically altered mouse models have become an integral part of research programs at Salk. The Transgenic Core is dedicated to providing access to cutting-age technologies to create these models. TG Core services include microinjection of DNA, CRISPR/Ca9 and lentiviral vectors into one-cell stage mouse embryos, microinjections of gene targeted mouse embryonic stem (ES) cells into blastocysts, cryopreservation and rederivation of mouse strains, in-vitro fertilization (IVF), gene targeting in mouse ES cells, targeting vectors design and construction, De novo derivation of ES cell lines and injections of human embryonic stem (hES) cells and induced pluripotent stem (iPS) cell lines into immunodeficient mice to form teratomas. In addition, the Core offers DR4 and CF1 mouse embryonic fibroblast (EF) cells, ES cell expansion and Southern Blot analyses.
The Transgenic Core develops new techniques and applications, provides immediate access to individuals with knowledge of dealing with transgenic and knock out mouse models, and offers the potential for research collaboration. More than 100 publications were based on genetically altered mouse lines created by Transgenic Core since 1994.
The Transgenic Core is part of the Salk Institute Cancer Center, directed by Dr. Reuben Shaw.
TG Core in located within pathogen-free barrier facility in which only animals from standard sources are allowed. The Core facility maintains a mouse colony in the three animals holding rooms adjacent to seven procedure rooms. The Institute ensures that all employees dealing with animals understand their individual and collective duties and embrace their ethical obligations to provide the highest level of care, conforming to all relevant regulations and rules concerning laboratory animal husbandry. All activities of the Transgenic Core facility are overseen by the Institutional Animal Care and Use Committee (IACUC), which is chaired by Dr. Paul Sawchenko.
National Cancer Institute
For submitting manuscripts that have made use of services or resources from this core, PLEASE ACKNOWLEDGE SUPPORT by inclusion of the following information:
“This work was supported by the Transgenic Core Facility of the Salk Institute with funding from NIH-NCI CCSG P30 CA014195.” Please acknowledge core staff where appropriate and consider authorship inclusion when scientific contributions merit.
Please link each publication stemming from work performed in the core to your MyNCBI account. This will ensure that the contributions from NIH are appropriately acknowledged in renewal and continuation applications. This NIH requirement must be satisfied as part of our efforts to provide consistent access to stabling funded core resources. Information on how to link publications can be found here»
10010 N Torrey Pines Rd
La Jolla, CA 92037
Telephone: (858) 453-4100
Access / Location
The TG Core Office is located on the main level of the Southeast building.
(858) 453-4100 x1937 or x1849
Yelena Dayn
Director
(858) 453-4100 x1937
dayn@salk.edu
Tsung-Chang Sung
Staff Researcher
(858) 453-4100 x1541
sung@salk.edu
