Diana Hargreaves, PhD

Associate Professor

Molecular and Cell Biology Laboratory

Salk Institute for Biological Studies - Publications

all Publications


Maxwell, M.B., Hom-Tedla, M.S., Yi, J., Li, S., Rivera, S.A., Yu, J., Burns, M.J., McRae, H.M., Stevenson, B.T., Coakley, K.E., Ho, J., Gastelum, K.B., Bell, J.C., Jones, A.C., Eskander, R.N., Dykhuizen, E.C., Shadel, G.S., Kaech, S.M., Hargreaves, D.C. ARID1A suppresses R-loop-mediated STING-type I interferon pathway activation of anti-tumor immunity. (2024) Cell. DOI: 10.1016/j.cell.2024.04.025

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Wu, Z., Pope, S.D., Ahmed, N.S., Leung, D.L., Hajjar, S., Yue, Q., Anand, D.M., Kopp, E.B., Okin, D., Ma, W., Kagan, J.C., Hargreaves, D.C., Medzhitov, R., Zhou, X. Control of Inflammatory Response by Tissue Microenvironment. (2024) bioRxiv. DOI: 10.1101/2024.05.10.592432

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Liao, J., Ho, J., Burns, M., Dykhuizen, E.C., Hargreaves, D.C. Collaboration between distinct SWI/SNF chromatin remodeling complexes directs enhancer selection and activation of macrophage inflammatory genes. (2024) Immunity. DOI: 10.1016/j.immuni.2024.05.008

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Liao, J., Hargreaves, D.C. Coordination of transcription factors and SWI-SNF complexes regulates chromatin priming in developing T cells. (2024) Nature Immunology. DOI: 10.1038/s41590-024-01822-z


Chick, B.Y., Hargreaves, D.C. RNA polymerase II promoter-proximal pausing promotes BAF chromatin binding and remodeling. (2023) Nature Genetics. DOI: 10.1038/s41588-023-01628-z

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McDonald, B., Chick, B.Y., Hargreaves, D.C., Kaech, S.M. Early Chromatin Remodeling Events in Acutely Stimulated CD8 T Cells. (2023) Yale J Biol Med. 96(4):467-473. DOI: 10.59249/AXGU7370

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Mangalhara, K.C., Varanasi, S.K., Johnson, M.A., Burns, M.J., Rojas, G.R., Esparza Moltó, P.B., Sainz, A.G., Tadepalle, N., Abbott, K.L., Mendiratta, G., Chen, D., Farsakoglu, Y., Kunchok, T., Hoffmann, F.A., Parisi, B., Rincon, M., Vander Heiden, M.G., Bosenberg, M., Hargreaves, D.C., Kaech, S.M., Shadel, G.S. Manipulating mitochondrial electron flow enhances tumor immunogenicity. (2023) Science. 381(6664):1316-1323. DOI: 10.1126/science.abq1053

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McDonald, B., Chick, B.Y., Ahmed, N.S., Burns, M., Ma, S., Casillas, E., Chen, D., Mann, T.H., O'Connor, C., Hah, N., Hargreaves, D.C., Kaech, S.M. Canonical BAF complex activity shapes the enhancer landscape that licenses CD8 T cell effector and memory fates. (2023) Immunity. 56(6):1303-1319.e5. DOI: 10.1016/j.immuni.2023.05.005

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Ferguson, L.P., Gatchalian, J., McDermott, M.L., Nakamura, M., Chambers, K., Rajbhandari, N., Lytle, N.K., Rosenthal, S.B., Hamilton, M., Albini, S., Wartenberg, M., Zlobec, I., Galván, J.A., Karamitopoulou, E., Vavinskaya, V., Wascher, A., Lowy, A.M., Schürch, C.M., Puri, P.L., Bruneau, B.G., Hargreaves, D.C., Reya, T. Smarcd3 is an epigenetic modulator of the metabolic landscape in pancreatic ductal adenocarcinoma. (2023) Nature Communications. 14(1):292. DOI: 10.1038/s41467-023-35796-7

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Chick, B.Y., Hargreaves, D.C. Switching under selection: how CoREST controls endocrine therapy resistance in ER breast cancer. (2022) Nature Structural & Molecular Biology. DOI: 10.1038/s41594-022-00858-9


Hishida, T., Yamamoto, M., Hishida-Nozaki, Y., Shao, C., Huang, L., Wang, C., Shojima, K., Xue, Y., Hang, Y., Shokhirev, M., Memczak, S., Sahu, S.K., Hatanaka, F., Ros, R.R., Maxwell, M.B., Chavez, J., Shao, Y., Liao, H.K., Martinez-Redondo, P., Guillen-Guillen, I., Hernandez-Benitez, R., Esteban, C.R., Qu, J., Holmes, M.C., Yi, F., Hickey, R.D., Garcia, P.G., Delicado, E.N., Castells, A., Campistol, J.M., Yu, Y., Hargreaves, D.C., Asai, A., Reddy, P., Liu, G.H., Izpisua Belmonte, J.C. In vivo partial cellular reprogramming enhances liver plasticity and regeneration. (2022) Cell Reports. 39(4):110730. DOI: 10.1016/j.celrep.2022.110730

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McRae, H.M., Hargreaves, D.C. Old macrophages lose their (circadian) rhythm. (2022) Trends in Immunology. DOI: 10.1016/j.it.2022.02.005

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Ahmed, N.S., Gatchalian, J., Ho, J., Burns, M.J., Hah, N., Wei, Z., Downes, M., Evans, R.M., Hargreaves, D.C. BRD9 regulates interferon-stimulated genes during macrophage activation via cooperation with BET protein BRD4. (2022) Proceedings of the National Academy of Sciences of the United States of America. 119(1). DOI: 10.1073/pnas.2110812119

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Maxwell, M.B., Hargreaves, D.C. Down, but Not Out: A Role for SMARCB1 in Synovial Sarcoma. (2021) Cancer Discovery. 11(10):2375-2377. DOI: 10.1158/2159-8290.CD-21-0591

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Wang, L., Oh, T.G., Magida, J., Estepa, G., Obayomi, S.M.B., Chong, L.W., Gatchalian, J., Yu, R.T., Atkins, A.R., Hargreaves, D., Downes, M., Wei, Z., Evans, R.M. Bromodomain containing 9 (BRD9) regulates macrophage inflammatory responses by potentiating glucocorticoid receptor activity. (2021) Proceedings of the National Academy of Sciences of the United States of America. 118(35). DOI: 10.1073/pnas.2109517118

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Hargreaves, D.C. Chromatin openness requires continuous SWI/SNF activity. (2021) Nature Genetics. DOI: 10.1038/s41588-021-00781-7


Liao, J., Hargreaves, D.C. The alternative macrophage relay: STAT6 passes the baton to EGR2. (2020) Genes & Development. 34(21-22):1407-1409. DOI: 10.1101/gad.345140.120

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Loo, C.S., Gatchalian, J., Liang, Y., Leblanc, M., Xie, M., Ho, J., Venkatraghavan, B., Hargreaves, D.C., Zheng, Y. A Genome-wide CRISPR Screen Reveals a Role for the Non-Canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function. (2020) Immunity. DOI: 10.1016/j.immuni.2020.06.011

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Cortez, J.T., Montauti, E., Shifrut, E., Gatchalian, J., Zhang, Y., Shaked, O., Xu, Y., Roth, T.L., Simeonov, D.R., Zhang, Y., Chen, S., Li, Z., Woo, J.M., Ho, J., Vogel, I.A., Prator, G.Y., Zhang, B., Lee, Y., Sun, Z., Ifergan, I., Van Gool, F., Hargreaves, D.C., Bluestone, J.A., Marson, A., Fang, D. CRISPR screen in regulatory T cells reveals modulators of Foxp3. (2020) Nature. DOI: 10.1038/s41586-020-2246-4

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Gatchalian, J., Liao, J., Maxwell, M.B., Hargreaves, D.C. Control of Stimulus-Dependent Responses in Macrophages by SWI/SNF Chromatin Remodeling Complexes. (2020) Trends in Immunology. DOI: 10.1016/j.it.2019.12.002

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Gao, F., Elliott, N.J., Ho, J., Sharp, A., Shokhirev, M.N., Hargreaves, D.C. Heterozygous Mutations in SMARCA2 Reprogram the Enhancer Landscape by Global Retargeting of SMARCA4. (2019) Molecular Cell. 75(5):891-904. DOI: 10.1016/j.molcel.2019.06.024

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Hargreaves, D.C. Tuning the chromatin landscape of embryonic stem cells. (2019) Stem Cell Investigation. 6:16. DOI: 10.21037/sci.2019.06.02


Gatchalian, J., Malik, S., Ho, J., Lee, D.S., Kelso, T.W.R., Shokhirev, M.N., Dixon, J.R., Hargreaves, D.C. A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells. (2018) Nature Communications. 9(1):5139. DOI: 10.1038/s41467-018-07528-9

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Gatchalian, J., Hargreaves, D.C. Racheting Up Repair. (2018) Immunity. 49(4):577-579. DOI: 10.1016/j.immuni.2018.09.012

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Marian, C.A., Stoszko, M., Wang, L., Leighty, M.W., de Crignis, E., Maschinot, C.A., Gatchalian, J., Carter, B.C., Chowdhury, B., Hargreaves, D.C., Duvall, J.R., Crabtree, G.R., Mahmoudi, T., Dykhuizen, E.C. Small Molecule Targeting of Specific BAF (mSWI/SNF) Complexes for HIV Latency Reversal. (2018) Cell Chemical Biology. DOI: 10.1016/j.chembiol.2018.08.004

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Kelso, T.W.R., Porter, D.K., Amaral, M.L., Shokhirev, M.N., Benner, C., Hargreaves, D.C. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. (2017) Elife. 6. DOI: 10.7554/eLife.30506

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Miller, E.L., Hargreaves, D.C., Kadoch, C., Chang, C.Y., Calarco, J.P., Hodges, C., Buenrostro, J.D., Cui, K., Greenleaf, W.J., Zhao, K., Crabtree, G.R. TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin. (2017) Nature Structural & Molecular Biology. 24(4):344-352. DOI: 10.1038/nsmb.3384

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Kadoch, C., Hargreaves, D.C., Hodges, C., Elias, L., Ho, L., Ranish, J., Crabtree, G.R. Proteomic and bioinformatic analysis of mammalian SWI/SNF complexes identifies extensive roles in human malignancy. (2013) Nature Genetics. 45(6):592-601. DOI: 10.1038/ng.2628

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Dykhuizen, E.C., Hargreaves, D.C., Miller, E.L., Cui, K., Korshunov, A., Kool, M., Pfister, S., Cho, Y.J., Zhao, K., Crabtree, G.R. BAF complexes facilitate decatenation of DNA by topoisomerase IIα. (2013) Nature. 497(7451):624-7. DOI: 10.1038/nature12146

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Hargreaves, D.C., Crabtree, G.R. ATP-dependent chromatin remodeling: genetics, genomics and mechanisms. (2011) Cell Research. 21(3):396-420. DOI: 10.1038/cr.2011.32

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Hargreaves, D.C., Horng, T., Medzhitov, R. Control of inducible gene expression by signal-dependent transcriptional elongation. (2009) Cell. 138(1):129-45. DOI: 10.1016/j.cell.2009.05.047

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Foster, S.L., Hargreaves, D.C., Medzhitov, R. Gene-specific control of inflammation by TLR-induced chromatin modifications. (2007) Nature. 447(7147):972-8. DOI: 10.1038/nature05836

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Hargreaves, D.C., Medzhitov, R. Innate sensors of microbial infection. (2005) J. Clin. Immunol. 25(6):503-10. DOI: 10.1007/s10875-005-8065-4

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Luther, S.A., Bidgol, A., Hargreaves, D.C., Schmidt, A., Xu, Y., Paniyadi, J., Matloubian, M., Cyster, J.G. Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis. (2002) Journal of immunology. 169(1):424-33.

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Cyster, J.G., Ansel, K.M., Ngo, V.N., Hargreaves, D.C., Lu, T.T. Traffic patterns of B cells and plasma cells. (2002) Advances in Experimental Medicine and Biology. 512:35-41.


Hargreaves, D.C., Hyman, P.L., Lu, T.T., Ngo, V.N., Bidgol, A., Suzuki, G., Zou, Y.R., Littman, D.R., Cyster, J.G. A coordinated change in chemokine responsiveness guides plasma cell movements. (2001) Journal of Experimental Medicine. 194(1):45-56.

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Education

BS, Chemistry and Biochemistry, Haverford College
PhD, Department of Immunobiology, Yale University
Postdoctoral Fellowship, Department of Developmental Biology, Stanford University


Awards & Honors

  • Pew-Stewart Scholar for Cancer Research, 2019
  • V Foundation for Cancer Research, V Foundation Scholar Award, 2016-2018
  • NCI/NIH K99/R00 Pathway to Independence Award, 2014-2018
  • Ovarian Cancer Research Fund, Ann Schreiber Research Training Program of Excellence, 2013-2014
  • Helen Hay Whitney Foundation Postdoctoral Fellowship, 2010-2013
  • National Science Foundation Graduate Research Fellowship, 2002-2005